RPAP3 Polyclonal antibody, PBS Only

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23741-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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Product Information

23741-1-PBS targets RPAP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPAP3 fusion protein Ag20661
• Full Name: RNA polymerase II associated protein 3
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC056415
• Gene Symbol: RPAP3
• Gene ID (NCBI): 79657
• RRID: AB_2879313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.