RPAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPAP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15138-1-AP

Synonyms

KIAA1403, RPAP1

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Validation Data Gallery

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  WB analysis using 15138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15138-1-AP (RPAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15138-1-AP

  WB result of RPAP1 antibody (15138-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPAP1 transfected HEK-293 cells.

• 

  IP experiment of HeLa using 15138-1-AP

  IP result of anti-RPAP1 (IP:15138-1-AP, 3ug; Detection:15138-1-AP 1:800) with HeLa cells lysate 3000ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15138-1-AP targets RPAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPAP1 fusion protein Ag7286
• Full Name: RNA polymerase II associated protein 1
• Calculated Molecular Weight: 153 kDa
• Observed Molecular Weight: 153 kDa
• GenBank Accession Number: BC000246
• Gene Symbol: RPAP1
• Gene ID (NCBI): 26015
• RRID: AB_2301137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

Publications

Species	Application	Title
mouse	WB	Cell Rep; The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.; Authors - Cian J Lynch ; KD Validated
human	WB	Cell Rep; Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome.; Authors - Isabel Pablos