RP2 Polyclonal antibody

• 

  IP experiment of Y79 using 14151-1-AP

  IP result of anti-RP2 (IP:14151-1-AP, 4ug; Detection:14151-1-AP 1:500) with Y79 cells lysate 2000ug.

• 

  IHC staining of human liver cancer using 14151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14151-1-AP (RP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14151-1-AP (RP2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14151-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2

---

Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, Y79 cells, mouse liver tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14151-1-AP targets RP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RP2 fusion protein Ag5332
• Full Name: retinitis pigmentosa 2 (X-linked recessive)
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC043348
• Gene Symbol: RP2
• Gene ID (NCBI): 6102
• RRID: AB_11182501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75695
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	IHC	Cell Mol Life Sci; A look into retinal organoids: methods, analytical techniques, and applications.; Authors - Tess A V Afanasyeva
mouse	WB	Sci Rep; The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia.; Authors - Stefan Kurtenbach