ROS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ROS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28500-1-AP

Synonyms

c ros 1, MCF3, Proto oncogene c Ros 1, ROS, ROS1

---

Validation Data Gallery

• 

  WB analysis of hela using 28500-1-AP

  WB result of ROS1 antibody (28500-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROS1 transfected HeLa cells.

• 

  WB analysis using 28500-1-AP

  HeLa lysates were subjected to SDS PAGE followed by western blot with 28500-1-AP (ROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28500-1-AP targets ROS1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROS1 fusion protein Ag28943
• Full Name: c-ros oncogene 1 , receptor tyrosine kinase
• Calculated Molecular Weight: 264 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_002944
• Gene Symbol: ROS1
• Gene ID (NCBI): 6098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (PMID: 3023956). It's reported that rearrangements of the ROS1 gene occur in many cancers. The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (PMID: 12661006), and subsequent studies have found this fusion in cholangiocarcinoma, ovarian cancer and non-small cell lung cancer (NSCLC) (PMID: 21253578, 22163003, 22661537). Additional oncogenic ROS1 fusion proteins have been found in NSCLC (at a frequency of 1-2%), including FIG, CD74, SLC34A2 and SDC4, and the list is growing. CD74-ROS1 is the most frequently detected ROS1 fusion in this group of patients. With all of the known fusion genes, the ROS1 kinase domain is fully retained and the ROS1 junction point at the messenger RNA (mRNA) level invariably occurs at the 5' end of exons 32, 34, 35 or 36 (PMID: 27535289). The molecular mass of some ROS1 fusion proteins are detected as follows: FIG-ROS1 (110 kDa), SLC34A2-ROS1 (85/70/59 kDa), CD74-ROS1 (80 kDa), and SDC4-ROS1 (75 kDa). (PMID: 22661537, 22659450)