RORC Polyclonal antibody

RORC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29910-1-AP

Synonyms

RORG, ROR gamma T, Retinoid-related orphan receptor-gamma, RAR-related orphan receptor C, Nuclear receptor ROR-gamma

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Validation Data Gallery

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  WB analysis using 29910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29910-1-AP (RORC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29910-1-AP targets RORC in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RORC fusion protein Ag31783
• Full Name: RAR-related orphan receptor C
• Calculated Molecular Weight: 518 aa, 58 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC031554
• Gene Symbol: RORC
• Gene ID (NCBI): 6097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Nuclear receptors retinoic acid receptor-related orphan receptor gamma (RORγ or RORc, also known as NR1F3) was the third and most recent ROR group member to be discovered, following the prior disclosures of RORα (RORa or NR1F1) and RORβ (RORb or NR1F2) (PMID:24502334). Studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2.RORC has 2 isoforms with the MW of 56 kDa and 58 kDa.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Aucklandiae radix targeted PKM2 to alleviate ulcerative colitis: Insights from the photocrosslinking target fishing technique; Authors - Yan-Li Feng
human	WB	Int Immunopharmacol; SLAMF3 promotes Th17 differentiation and is reversed by iguratimod through JAK1/STAT3 pathway in primary Sjögren's syndrome; Authors - Peini Hu