RORB Polyclonal antibody

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  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 17635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17635-1-AP

  IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.

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  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17635-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB

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Tested Applications

• Positive WB detected in: Y79 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below

Product Information

17635-1-AP targets RORB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RORB fusion protein Ag11910
• Full Name: RAR-related orphan receptor B
• Calculated Molecular Weight: 459 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051830
• Gene Symbol: RORB
• Gene ID (NCBI): 6096
• RRID: AB_2180290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Publications

Species	Application	Title
mouse	IF	Neuron; Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.; Authors - Chao Wang
mouse	IF	PLoS Biol; An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold; Authors - Wenny Wong
mouse	IF	eNeuro; Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.; Authors - Natalia Galindo-Riera

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)