RON, MST1R Monoclonal antibody

RON, MST1R Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8D1

Cat no : 67954-1-Ig

Synonyms

CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON

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Validation Data Gallery

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  WB analysis of BxPC-3 using 67954-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67954-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67954-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 67954-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67954-1-Ig targets RON, MST1R in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RON, MST1R fusion protein Ag28289
• Full Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• Calculated Molecular Weight: 152 kDa
• Observed Molecular Weight: 150-185 kDa
• GenBank Accession Number: BQ936677 NM_002447
• Gene Symbol: RON
• Gene ID (NCBI): 4486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.