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ROCK1 Recombinant antibody, PBS Only

ROCK1 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230423C6

Cat no : 83166-5-PBS

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ROCK1 Recombinant antibody (83166-5-RR)

    WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ROCK1 Recombinant antibody (83166-5-RR)

    WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ROCK1 Recombinant antibody (83166-5-RR)

    IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

  • Affinity experiments of 83166-5-RR

    Affinity experiments of 83166-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83166-5-PBS is ROCK1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ROCK1 fusion protein Ag16317
Full Name Rho-associated, coiled-coil containing protein kinase 1
Calculated Molecular Weight 1354 aa, 158 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.