ROCK1 Recombinant antibody, PBS Only

ROCK1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230423C6

Cat no : 83166-5-PBS

Synonyms

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 83166-5-RR (same clone as 83166-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83166-5-RR (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (83166-5-RR, Clone: 230423C6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83166-5-RR (same clone as 83166-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ROCK1 Recombinant antibody (83166-5-RR, Clone:230423C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83166-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83166-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83166-5-RR against Human ROCK1 were performed. The affinity constant is 0.401 nM.

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Product Information

The immunogen of 83166-5-PBS is ROCK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ROCK1 fusion protein Ag16317
• Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Calculated Molecular Weight: 1354 aa, 158 kDa
• GenBank Accession Number: BC113114
• Gene Symbol: ROCK1
• Gene ID (NCBI): 6093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.