ROCK1 Recombinant antibody, PBS Only (Capture)

ROCK1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230423H5

Cat no : 83166-4-PBS

Synonyms

Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1, EC:2.7.11.1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00229-3

  Sandwich ELISA standard curve of MP00229-3, Human ROCK1 Recombinant Matched Antibody Pair - PBS only. 83166-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16317. 83166-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00229-3

  The mean ROCK1 concentration was determined to be 4,443.0 pg/mL in A431 cell extract based on a 2.6 mg/mL extract load and 13,265.0 pg/mL in HepG2 cell extract based on a 5.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP00229-1

  Cytometric bead array standard curve of MP00229-1, ROCK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83166-4-PBS. Detection antibody: 83166-2-PBS. Standard: Ag16317. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83166-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83166-4-PBS against Human ROCK1 was performed. The affinity constant is 1.56 nM.

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Product Information

83166-4-PBS targets ROCK1 as part of a matched antibody pair:

MP00229-1: 83166-4-PBS capture and 83166-2-PBS detection (validated in Cytometric bead array)

MP00229-3: 83166-4-PBS capture and 83166-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ROCK1 fusion protein Ag16317
• Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Calculated Molecular Weight: 1354 aa, 158 kDa
• GenBank Accession Number: BC113114
• Gene Symbol: ROCK1
• Gene ID (NCBI): 6093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.