ROBO3 Polyclonal antibody

ROBO3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11982-1-AP

Synonyms

HGPPS, HGPS, RBIG1, RIG1, ROBO3, Roundabout homolog 3, Roundabout like protein 3

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Validation Data Gallery

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  WB analysis using 11982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11982-1-AP (ROBO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11982-1-AP targets ROBO3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROBO3 fusion protein Ag2608
• Full Name: roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 200-250 kDa, 148 kDa
• GenBank Accession Number: BC008623
• Gene Symbol: ROBO3
• Gene ID (NCBI): 64221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS).