ROBO1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

ROBO1-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 20219-1-AP

Synonyms

Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, ROBO1-Specific, Roundabout homolog 1, SAX3

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Validation Data Gallery

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  WB analysis using 20219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20219-1-AP (ROBO1-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

20219-1-AP targets ROBO1-Specific in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_002941
• Gene Symbol: ROBO1
• Gene ID (NCBI): 6091
• RRID: AB_10694675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development. The antibody is specific to ROBO1.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB	Mol Cell; AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.; Authors - Hana Cho ; KD Validated
human	IF	Chin Med J (Engl); Repression of Smad3 by silencing Robo1 attenuates epithelial-mesenchymal transition in tracheobronchial stenosis; Authors - Chunyan He
human	WB	Aging (Albany NY); MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.; Authors - Zongpu Zhang ; KD Validated
human	WB	Angiogenesis; Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.; Authors - Junhui Liu
human	WB	iScience; Activation of EPHA2-ROBO1 Heterodimer by SLIT2 Attenuates Non-canonical Signaling and Proliferation in Squamous Cell Carcinomas.; Authors - Saumya Srivastava ; KD Validated