ROBO1 Polyclonal antibody

ROBO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25181-1-AP

Synonyms

Deleted in U twenty twenty, DUTT1, H Robo 1, ROBO1, Roundabout homolog 1, SAX3

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Validation Data Gallery

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  WB analysis of Y79 using 25181-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25181-1-AP (ROBO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25181-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25181-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25181-1-AP (ROBO1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Y79 cells, HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25181-1-AP is ROBO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROBO1 fusion protein Ag16461
• Full Name: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• Calculated Molecular Weight: 1651 aa, 181 kDa
• Observed Molecular Weight: 200-250 kDa
• GenBank Accession Number: BC112336
• Gene Symbol: ROBO1
• Gene ID (NCBI): 6091
• RRID: AB_2879946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.