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RNMT Monoclonal antibody, PBS Only

RNMT Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F1C9

Cat no : 67673-1-PBS

Synonyms

DKFZp686H1252, hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, MET, mRNA cap methyltransferase, RG7MT1, RNMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNMT Monoclonal antibody (67673-1-Ig)

    WB analysis using 67673-1-Ig (same clone as 67673-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67673-1-Ig (RNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67673-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67673-1-PBS targets RNMT in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RNMT fusion protein Ag6953
Full Name RNA (guanine-7-) methyltransferase
Calculated Molecular Weight 476 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC036798
Gene Symbol RNMT
Gene ID (NCBI) 8731
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.