RNMT Polyclonal antibody

• Featured Product
• KD/KO Validated

RNMT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 13743-1-AP

Synonyms

KIAA0398, hMet, hCMT1c, hCMT1, hcm1p

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Validation Data Gallery

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  WB analysis of human kidney using 13743-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13743-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13743-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 13743-1-AP

  IP result of anti-RNMT (IP:13743-1-AP, 4ug; Detection:13743-1-AP 1:3000) with L02 cells lysate 1040 ug.

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  IHC staining of human colon cancer using 13743-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13743-1-AP (RNMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 13743-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13743-1-AP (RNMT antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13743-1-AP

  Immunofluorescent analysis of Hela cells, using RNMT antibody 13743-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human kidney tissue, HeLa cells, human liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Product Information

13743-1-AP targets RNMT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNMT fusion protein Ag4682
• Full Name: RNA (guanine-7-) methyltransferase
• Calculated Molecular Weight: 476 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036798
• Gene Symbol: RNMT
• Gene ID (NCBI): 8731
• RRID: AB_2181550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.

Publications

Species	Application	Title
human	WB	Cell; RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.; Authors - Fustin Jean-Michel JM ; KD Validated
human	WB	Nucleic Acids Res; Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.; Authors - Daniel Del Valle Morales
human	WB,CoIP	J Mol Biol; Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.; Authors - Michael J Osborne
human	WB	Heliyon; RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light; Authors - Lenka Stixová
human	WB	ACS Chem Biol; Comparative Analysis of Two NNMT Bisubstrate Inhibitors through Chemoproteomic Studies: Uncovering the Role of Unconventional SAM Analogue Moiety for Improved Selectivity; Authors - Ying Meng
human	WB	Immunobiology; Identification of RNMT as an immunotherapeutic and prognostic biomarker: From pan-cancer analysis to lung squamous cell carcinoma validation; Authors - Shuqiang Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karthik Subramanian (Verified Customer) (07-18-2019)

The antibody initially worked very well for my western blot applications. Subsequently, however, its performance has considerably become poorer and often results in fainter bands with some amount of non-specificity.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa