RNH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10345-1-AP

Synonyms

RNH, Ribonuclease/angiogenin inhibitor 1, Ribonuclease inhibitor, RAI, PRI

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Validation Data Gallery

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10345-1-AP

  WB result of RNH1 antibody (10345-1-AP, 1:2500) with si-control and si-RNH1 transfected HeLa cells.

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10345-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10345-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10345-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10345-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IP experiment of HEK-293T using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with HEK-293T cells lysate 2040 ug.

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  IP experiment of mouse liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

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  IP experiment of rat liver using 10345-1-AP

  IP result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with rat liver tissue lysate 5520 ug.

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10345-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RNH1 antibody (10345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10345-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RNH1 (10345-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, human brain tissue, human heart tissue, human liver tissue, L02 cells, HepG2 cells, Jurkat cells, human placenta tissue
• Positive IP detected in: mouse liver tissue, HEK-293T cells, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

10345-1-AP targets RNH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNH1 fusion protein Ag0350
• Full Name: ribonuclease/angiogenin inhibitor 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC000677
• Gene Symbol: RNH1
• Gene ID (NCBI): 6050
• RRID: AB_2269733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Publications

Species	Application	Title
mouse	WB	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
human	WB	J Cell Biol; Angiogenin cleaves tRNA and promotes stress-induced translational repression.; Authors - Yamasaki Satoshi S ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.; Authors - Shozo Honda
human	WB	Int J Mol Sci; RTCB Complex Regulates Stress-Induced tRNA Cleavage; Authors - Yasutoshi Akiyama
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
mouse	WB	J Biol Chem; Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.; Authors - Saikia Mridusmita M