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RNF8 Monoclonal antibody, PBS Only

RNF8 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G3H5

Cat no : 68521-1-PBS

Synonyms

FLJ12013, KIAA0646, ring finger protein 8, RNF8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF8 Monoclonal antibody (68521-1-Ig)

    WB analysis using 68521-1-Ig (same clone as 68521-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68521-1-Ig (RNF8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68521-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68521-1-PBS targets RNF8 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RNF8 fusion protein Ag5292
Full Name ring finger protein 8
Calculated Molecular Weight 485 aa, 56 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC007517
Gene Symbol RNF8
Gene ID (NCBI) 9025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.