RNF8 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RNF8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14112-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

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  WB analysis using 14112-1-AP (same clone as 14112-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14112-1-AP (same clone as 14112-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

  IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF8 antibody (14112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

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Product Information

14112-1-PBS targets RNF8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF8 fusion protein Ag5292
• Full Name: ring finger protein 8
• Calculated Molecular Weight: 485 aa, 56 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC007517
• Gene Symbol: RNF8
• Gene ID (NCBI): 9025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.