RNF7 Polyclonal antibody

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  WB analysis of human liver using 11905-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11905-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11905-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11905-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11905-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11905-1-AP

  IP result of anti-RNF7 (IP:11905-1-AP, 4ug; Detection:11905-1-AP 1:600) with mouse heart tissue lysate 4400ug.

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  IF Staining of mouse heart using 11905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RNF7 antibody (11905-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 11905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RNF7 antibody (11905-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

RNF7 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Cat No. 11905-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

SAG, ROC2, RING-box protein 2, RBX2, CKII beta-binding protein 1

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Tested Applications

• Positive WB detected in: human liver tissue, human heart tissue, human skeletal muscle tissue, human testis tissue
• Positive IP detected in: mouse heart tissue
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11905-1-AP targets RNF7 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF7 fusion protein Ag2553
• Full Name: ring finger protein 7
• Calculated Molecular Weight: 113 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC008627
• Gene Symbol: RNF7
• Gene ID (NCBI): 9616
• RRID: AB_10697836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBF6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.

Publications

Species	Application	Title
human	WB	EMBO J; RHEB neddylation by the UBE2F-SAG axis enhances mTORC1 activity and aggravates liver tumorigenesis; Authors - Fengwu Zhang
human	WB	Mol Cell Proteomics; Neddylation-Cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer.; Authors - Junfeng Xu ; KD Validated
human	WB	Dev Cell; The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells; Authors - Yu Chang
human,mouse	IHC	Nat Aging; Cell type mapping of inflammatory muscle diseases highlights selective myofiber vulnerability in inclusion body myositis; Authors - Sven Wischnewski
human	WB	FASEB J; PMID: 30844312; Authors - Tengyu Ko ; KD Validated