RNF41 Polyclonal antibody

RNF41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17233-1-AP

Synonyms

FLRF, NRDP1, ring finger protein 41, RNF41, SBBI03

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Validation Data Gallery

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  WB analysis of human testis using 17233-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17233-1-AP (RNF41 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17233-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17233-1-AP (RNF41 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17233-1-AP (RNF41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17233-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17233-1-AP (RNF41 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17233-1-AP is RNF41 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF41 fusion protein Ag11076
• Full Name: ring finger protein 41
• Calculated Molecular Weight: 317 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC032637
• Gene Symbol: RNF41
• Gene ID (NCBI): 10193
• RRID: AB_2878365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,rat	WB	Front Cardiovasc Med; Deficiency of a novel lncRNA-HRAT protects against myocardial ischemia reperfusion injury by targeting miR-370-3p/RNF41 pathway; Authors - Xinbin Zheng