RNF31 / HOIP Polyclonal antibody

RNF31 / HOIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16289-1-AP

Synonyms

HOIP, RNF31, HOIL-1-interacting protein, HOIL 1 interacting protein, FLJ23501

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Validation Data Gallery

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  WB analysis of HeLa using 16289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16289-1-AP (HOIP antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected cell cell using 16289-1-AP

  WB result of RNF31/HOIP antibody (16289-1-AP) by Emmanuel Laplantine (Institute Pasteur,Paris,France).

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  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Transfected cell cell
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

16289-1-AP targets RNF31 / HOIP in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF31 / HOIP fusion protein Ag9440
• Full Name: ring finger protein 31
• Calculated Molecular Weight: 1072 aa, 120 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC017376
• Gene Symbol: RNF31
• Gene ID (NCBI): 55072
• RRID: AB_2878239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)

Publications

Species	Application	Title
human	WB	Oncogene; AGO4 suppresses tumor growth by modulating autophagy and apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in a p53-independent manner; Authors - Lin Wang
mouse	WB	J Immunol; HOIL1 Regulates Group 3 Innate Lymphoid Cells in the Colon and Protects against Systemic Dissemination, Colonic Ulceration, and Lethality from Citrobacter rodentium Infection; Authors - Victoria L Hartley
human	WB	Bioorg Med Chem; Biophysical and biological evaluation of optimized stapled peptide inhibitors of the linear ubiquitin chain assembly complex (LUBAC).; Authors - Francisco Aguilar-Alonso
human	WB	BMC Musculoskelet Disord; Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.; Authors - Lin Chen
human	WB	Arch Oral Biol; A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.; Authors - Shichen Sun
human	WB	Cancer Commun (Lond); USP51/PD-L1/ITGB1-deployed juxtacrine interaction plays a cell-intrinsic role in promoting chemoresistant phenotypes in non-small cell lung cancer; Authors - Jianjun Li