RNF217 Polyclonal antibody, PBS Only

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  IHC staining of human liver using 26084-1-AP (same clone as 26084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 26084-1-AP (same clone as 26084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 26084-1-AP (same clone as 26084-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF217 antibody (26084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

RNF217 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 26084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

26084-1-PBS targets RNF217 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF217 fusion protein Ag22022
• Full Name: ring finger protein 217
• Calculated Molecular Weight: 275 aa, 32 kDa
• Observed Molecular Weight: 59, 32 kDa
• GenBank Accession Number: BC026087
• Gene Symbol: RNF217
• Gene ID (NCBI): 154214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TC41
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

E3 ubiquitin-protein ligase RNF217, also known as IBR domain-containing protein 1 (IBRDC1), as a transmembrane (TM) domain-containing RBR-type E3 ubiquitin-protein ligase, belongs to a highly conserved member of the RING finger family. RNF217 mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis(Uniprot, PMID: 33895792).