RNF213 Polyclonal antibody, PBS Only

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  WB analysis using 21028-1-AP (same clone as 21028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21028-1-AP (RNF213 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

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  IP experiment of HUVEC using 21028-1-AP (same clone as 21028-1-PBS)

  IP result of anti-RNF213 (IP:21028-1-AP, 4ug; Detection:21028-1-AP 1:300) with HUVEC cells lysate 910 ug. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21028-1-AP (same clone as 21028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21028-1-AP (same clone as 21028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 21028-1-AP (same clone as 21028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 21028-1-AP (same clone as 21028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21028-1-PBS in a different storage buffer formulation.

RNF213 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 21028-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ring finger protein 213, Protein ALO17, KIAA1618, KIAA1554, C17orf27

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Product Information

21028-1-PBS targets RNF213 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF213 fusion protein Ag15335
• Full Name: ring finger protein 213
• Calculated Molecular Weight: 3280 aa, 374 kDa
• GenBank Accession Number: BC032220
• Gene Symbol: RNF213
• Gene ID (NCBI): 57674
• RRID: AB_2878793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q63HN8
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNF213 is a typical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PMID: 21799892, 26126547, 26278786, 26766444, 30705059).RNF213 contains a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions.