RNF181 Polyclonal antibody, PBS Only

RNF181 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20408-1-PBS

Synonyms

RN181, RING finger protein 181, HSPC238, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF181

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Validation Data Gallery

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  WB analysis of Jurkat using 20408-1-AP (same clone as 20408-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 20408-1-AP (same clone as 20408-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 20408-1-AP (same clone as 20408-1-PBS)

  IP result of anti-RNF181 (IP:20408-1-AP, 3ug; Detection:20408-1-AP 1:500) with Jurkat cells lysate 1200ug. This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20408-1-AP (same clone as 20408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20408-1-AP (same clone as 20408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20408-1-AP (same clone as 20408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20408-1-AP (same clone as 20408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20408-1-AP (same clone as 20408-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF181 antibody (20408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20408-1-PBS in a different storage buffer formulation.

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Product Information

20408-1-PBS targets RNF181 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF181 fusion protein Ag14233
• Full Name: ring finger protein 181
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC002803
• Gene Symbol: RNF181
• Gene ID (NCBI): 51255
• RRID: AB_10696314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.