RNF17 Polyclonal antibody

RNF17 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24302-1-AP

Synonyms

Mmip 2, ring finger protein 17, RNF17, SPATA23, TDRD4

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Validation Data Gallery

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  WB analysis of human testis using 24302-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 24302-1-AP (RNF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24302-1-AP

  IP result of anti-RNF17 (IP:24302-1-AP, 4ug; Detection:24302-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 24302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24302-1-AP (RNF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24302-1-AP (RNF17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 24302-1-AP is RNF17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF17 fusion protein Ag20144
• Full Name: ring finger protein 17
• Calculated Molecular Weight: 1623 aa, 185 kDa
• Observed Molecular Weight: 184 kDa
• GenBank Accession Number: BC146822
• Gene Symbol: RNF17
• Gene ID (NCBI): 56163
• RRID: AB_2879484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF17 also termed as ring finger protein 17 is a 1623 amino acid protein, which contains 1 ring type zinc finger and 4 tudor domains. RNF17 is specifically expressed in testis. The RING finger motif presents in many ubiquitin E3 ligases. RNF17 interacts with all four members of the Mad family (Mad1, Mxi1, Mad3 and Mad4), which are basic-helix-loop-helix-leucine zipper transcription factors of the Myc oncoprotein network. RNF17 is component of the tectonic-like complex, which is required for ciliogenesis and sonic hedgehog/SHH signaling. RNF17 is able to form dimers or polymers both in vitro and in vivo, indicating that it may play a role in the assembly of RNF17 granules. RNF17 encodes a novel key regulator of spermiogenesis.

Publications

Species	Application	Title
mouse	WB,IF	PLoS Genet; Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association; Authors - Lauren G Chukrallah
human	IF	Andrology; Homozygous deleterious variants in the C-terminal of TDRD5 impair spermiogenesis, causing severe oligoasthenoteratozoospermia in humans; Authors - Rui Guo