RNF167 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF167 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24618-1-AP

Synonyms

5730408C10Rik, LP2254, ring finger protein 167, RING105, RNF167

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 24618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24618-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 24618-1-AP is RNF167 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF167 fusion protein Ag18383
• Full Name: ring finger protein 167
• Calculated Molecular Weight: 350 aa, 38 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC010139
• Gene Symbol: RNF167
• Gene ID (NCBI): 26001
• RRID: AB_2879642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

Publications

Species	Application	Title
human	WB	Nat Commun; RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.; Authors - Tingting Li ; KD Validated
human	WB	FASEB J; RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation; Authors - Zhenzhen Yan