RNF144A Polyclonal antibody

RNF144A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26144-1-AP

Synonyms

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

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Validation Data Gallery

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  WB analysis of MDA-MB-231 using 26144-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours.

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  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug.

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  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3080ug.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells
• Positive IP detected in: CHO cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26144-1-AP targets RNF144A in WB, IP, ELISA applications and shows reactivity with human, hamster samples.

• Tested Reactivity: human, hamster
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF144A fusion protein Ag23508
• Full Name: ring finger protein 144A
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC050373
• Gene Symbol: RNF144A
• Gene ID (NCBI): 9781
• RRID: AB_2880402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).

Publications

Species	Application	Title
human	WB	EMBO Rep; RNF144A promotes antiviral responses by modulating STING ubiquitination; Authors - Bo Yang