RNF139 Recombinant antibody, PBS Only

RNF139 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240481G6

Cat no : 83755-2-PBS

Synonyms

RING finger protein 139, RCA1, HRCA1, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF139

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Validation Data Gallery

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  WB analysis using 83755-2-RR (same clone as 83755-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83755-2-RR (RNF139 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83755-2-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83755-2-RR (same clone as 83755-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Rnf139 Recombinant Antibody (83755-2-RR, Clone:240481G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83755-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83755-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83755-2-RR against Human RNF139 were performed. The affinity constant is 102 nM.

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Product Information

The immunogen of 83755-2-PBS is RNF139 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF139 fusion protein Ag21530
• Full Name: ring finger protein 139
• Calculated Molecular Weight: 664 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC064636
• Gene Symbol: RNF139
• Gene ID (NCBI): 11236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The RNF139 gene (ring-finger protein 139), also known as TRC8 (translocation in renal carcinoma from chromosome 8), was first identified from a family with 3;8 chromosomal translocation and hereditary renal cell carcinoma and thyroid cancer (PMID:22689053). RNF139 encodes an endoplasmic reticulum (ER) protein with 10 transmembrane segments that contain a sterol-sensing domain and a RING finger motif encoding an E3 ubiquitin ligase (PMID: 19706601). Northern blot and dot blot show that RNF139 is highly expressed in the testis, placenta, and adrenal gland and is expressed at lower levels in the heart, brain, liver, skeletal muscle, and pancreas (PMID: 9689122). It is reported that TRC8 suppresses tumorigenesis by targeting heme oxygenase-1 (HO-1), an antioxidant enzyme highly expressed in various cancers, for ubiquitination and degradation (PMID:22689053). TRC8 is also involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs) (PMID:17016439). Acetylation, phosphorylation, and autoubiquitination are common post-translational modifications of FNF139 protein.