RNF133 Recombinant antibody

RNF133 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240397C12

Cat no : 83462-1-RR

Synonyms

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12

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Validation Data Gallery

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  WB analysis using 83462-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83462-1-RR (RNF133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83462-1-RR against Human RNF133 were performed. The affinity constant is 0.714 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83462-1-RR is RNF133 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF133 fusion protein Ag14537
• Full Name: ring finger protein 133
• Calculated Molecular Weight: 376 aa, 42 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC022038
• Gene Symbol: RNF133
• Gene ID (NCBI): 168433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.