RNF125 Polyclonal antibody

RNF125 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 13290-1-AP

Synonyms

ring finger protein 125, RNF125, TRAC 1, TRAC1

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Validation Data Gallery

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  WB analysis of A2780 using 13290-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13290-1-AP (RNF125 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 13290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A2780 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

RNF125 expresses at a very low level in most cells and tissues.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13290-1-AP targets RNF125 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF125 fusion protein Ag3908
• Full Name: ring finger protein 125
• Calculated Molecular Weight: 232 aa, 26 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC012021
• Gene Symbol: RNF125
• Gene ID (NCBI): 54941
• RRID: AB_10597852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncol Lett; RNF125‑mediated ubiquitination of MCM6 regulates the proliferation of human liver hepatocellular carcinoma cells; Authors - Xueyi Feng
human	WB	Cell Rep; Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner; Authors - Xianghui Kong
human	WB,IHC	Oncogene; RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway; Authors - Zhigang Feng ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jessica (Verified Customer) (01-31-2019)

Works well to detect overexpression of RNF125 in HEK293TCan barely detect RNF125 in HCT116 cells after exposure to 10h SeV.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, HCT116