RNF10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16936-1-AP

Synonyms

RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10

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Validation Data Gallery

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  WB analysis using 16936-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 16936-1-AP

  IP result of anti-RNF10 (IP:16936-1-AP, 4ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 1480 ug.

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16936-1-AP

  Immunofluorescent analysis of HepG2 cells, using RNF10 antibody 16936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: PC-3 cells, Jurkat cells, human brain tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF10 fusion protein Ag10433
• Full Name: ring finger protein 10
• Calculated Molecular Weight: 811 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC016622
• Gene Symbol: RNF10
• Gene ID (NCBI): 9921
• RRID: AB_2181846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

Publications

Species	Application	Title
human	WB,IF	Elife; Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.; Authors - Margarita C Dinamarca ; KD Validated
human	WB	Cell Rep; The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.; Authors - Aitor Garzia ; KO Validated
mouse,human	WB	Nat Cell Biol; Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition; Authors - Weiwei Cui
mouse		J Clin Invest; RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication; Authors - Joonyoung Her
rat	WB,IHC	Mol Neurobiol; The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology.; Authors - Nicolò Carrano