RND2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RND2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13844-1-AP

Synonyms

ARHN, Rho family GTPase 2, RHO7, RhoN, RND2

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Validation Data Gallery

• 

  WB analysis of mouse uterus using 13844-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 13844-1-AP (RND2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13844-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13844-1-AP (RND2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 13844-1-AP

  IP result of anti-RND2 (IP:13844-1-AP, 3ug; Detection:13844-1-AP 1:300) with PC-3 cells lysate 1040ug.

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Tested Applications

• Positive WB detected in: mouse uterus tissue, PC-3 cells
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

13844-1-AP targets RND2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RND2 fusion protein Ag4807
• Full Name: Rho family GTPase 2
• Calculated Molecular Weight: 227 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC018096
• Gene Symbol: RND2
• Gene ID (NCBI): 8153
• RRID: AB_2181817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	J Clin Invest; Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice.; Authors - Casazza Andrea A ; KD Validated
mouse	WB,IHC	Mol Psychiatry; The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis.; Authors - Thomas Kerloch ; KO Validated
human	WB,IHC,IF	J Exp Clin Cancer Res; RND2 attenuates apoptosis and autophagy in glioblastoma cells by targeting the p38 MAPK signalling pathway.; Authors - Yang Xu ; KD Validated
mouse	WB,IF	Mol Biol Cell; Rnd2 differentially regulates oligodendrocyte myelination at different developmental periods; Authors - Yuki Miyamoto ; KD Validated