RNASET2 Recombinant antibody

RNASET2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240581C4

Cat no : 83650-1-RR

Synonyms

RNase T2, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19, bA514O12.3

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Validation Data Gallery

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  WB analysis using 83650-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83650-1-RR (RNASET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83650-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83650-1-RR against Human RNASET2 were performed. The affinity constant is 2.09 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83650-1-RR is RNASET2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNASET2 fusion protein Ag28353
• Full Name: ribonuclease T2
• Calculated Molecular Weight: 256 aa, 29 kDa
• Observed Molecular Weight: 25-45 kDa
• GenBank Accession Number: BC039713
• Gene Symbol: RNase T2
• Gene ID (NCBI): 8635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).