RNASET2 Polyclonal antibody, PBS Only

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  IP experiment of BxPC-3 using 13753-1-AP (same clone as 13753-1-PBS)

  IP result of anti-RNase T2 (IP:13753-1-AP, 4ug; Detection:13753-1-AP 1:300) with BxPC-3 cells lysate 4000ug. This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 13753-1-AP (same clone as 13753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 13753-1-AP (same clone as 13753-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

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  IF Staining of transfected cells using 13753-1-AP (same clone as 13753-1-PBS)

  IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

RNASET2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13753-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19

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Product Information

13753-1-PBS targets RNASET2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASET2 fusion protein Ag4695
• Full Name: ribonuclease T2
• Calculated Molecular Weight: 256 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC039713
• Gene Symbol: RNase T2
• Gene ID (NCBI): 8635
• RRID: AB_2285339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00584
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).