RNASEH2A Monoclonal antibody

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  WB analysis using 67976-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67976-1-Ig (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 67976-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67976-1-Ig (RNASEH2A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNASEH2A Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67976-1-Ig

Clone No.1A2E8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ribonuclease H2, subunit A, Ribonuclease H2 subunit A, JUNB, EC:3.1.26.4, Aicardi-Goutieres syndrome 4 protein

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Tested Applications

• Positive WB detected in: LNCaP cells, Hela cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67976-1-Ig targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RNASEH2A fusion protein Ag9256
• Full Name: ribonuclease H2, subunit A
• Calculated Molecular Weight: 299 aa, 33 kDa
• GenBank Accession Number: BC011748
• Gene Symbol: RNASEH2A
• Gene ID (NCBI): 10535
• RRID: AB_2918725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75792
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).