RNASE6 Polyclonal antibody, PBS Only

RNASE6 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16425-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of human liver using 16425-1-AP (same clone as 16425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16425-1-AP (same clone as 16425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 16425-1-AP (same clone as 16425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 16425-1-AP (same clone as 16425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 16425-1-AP (same clone as 16425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RNASE6 antibody (16425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

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Product Information

16425-1-PBS targets RNASE6 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASE6 fusion protein Ag9667
• Full Name: ribonuclease, RNase A family, k6
• Calculated Molecular Weight: 150 aa, 17 kDa
• GenBank Accession Number: BC020848
• Gene Symbol: RNASE6
• Gene ID (NCBI): 6039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rnase6, a protein belonging to the superfamily, is considered the only vertebrate specific enzyme known to have a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction, and host defense. Rnase6 expression was upregulated in the peripheral blood and plaque tissues of AS patients (PMID: 34395402).