RMND5B Polyclonal antibody

RMND5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 25803-1-AP

Synonyms

Protein RMD5 homolog B, RMND5B

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Validation Data Gallery

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  WB analysis of A549 using 25803-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25803-1-AP (RMND5B Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human colon using 25803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 25803-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 25803-1-AP

  Immunofluorescent analysis of A549 cells using 25803-1-AP (RMND5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human colon tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 25803-1-AP is RMND5B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RMND5B fusion protein Ag22714
• Full Name: required for meiotic nuclear division 5 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 393 aa, 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC009911
• Gene Symbol: RMND5B
• Gene ID (NCBI): 64777
• RRID: AB_2880243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (11-22-2021)

This antibody is quite good. I used it for KO validation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

RMND5B Antibody Western Blot validation (1:1000 dilution) in (Cat no:25803-1-AP)