RMI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RMI1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14630-1-AP

Synonyms

BLAP75, C9orf76, FAAP75, RMI1, RP11 346I8.1

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Validation Data Gallery

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  WB analysis using 14630-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14630-1-AP (RMI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14630-1-AP

  WB result of RMI1 antibody (14630-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RMI1 transfected HepG2 cells.

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  IP experiment of HeLa using 14630-1-AP

  IP result of anti-RMI1 (IP:14630-1-AP, 3ug; Detection:14630-1-AP 1:1500) with HeLa cells lysate 3800ug.

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  IHC staining of human lung cancer using 14630-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14630-1-AP (RMI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14630-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14630-1-AP (RMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Product Information

14630-1-AP targets RMI1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RMI1 fusion protein Ag6208
• Full Name: RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC064937
• Gene Symbol: RMI1
• Gene ID (NCBI): 80010
• RRID: AB_2301203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)

Publications

Species	Application	Title
human	WB	Mol Cell; Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.; Authors - Thomas J Nicholls ; KD Validated
human	WB	Nat Commun; Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.; Authors - Martin Moder ; KO Validated
human	WB	J Biol Chem; DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA-end resection in human cells.; Authors - Andreas Sturzenegger ; KD Validated
human	WB	J Cancer; Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p.; Authors - Jin Qian
human	WB	DNA Cell Biol; Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure.; Authors - Yuxiao Sun
human	WB	Int J Mol Sci; Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis.; Authors - Chang Xu