Phospho-RIPK1 (Ser166) Polyclonal antibody

Phospho-RIPK1 (Ser166) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 28252-1-AP

Synonyms

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser166), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

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Validation Data Gallery

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  WB analysis using 28252-1-AP

  Non-treated HT-29 and TNF alpha (HZ-1014) treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28252-1-AP (Phospho-RIPK1 (Ser166) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: TNF-alpha treated HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

28252-1-AP targets Phospho-RIPK1 (Ser166) in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_003804
• Gene Symbol: RIPK1
• Gene ID (NCBI): 8737
• RRID: AB_2881098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis; Authors - Huiling Zhang
human	WB	Cell Death Discov; LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening.; Authors - Lulu Wo
human	WB	Inflamm Bowel Dis; Phosphoproteomics Reveals Novel Insights into the Pathogenesis and Identifies New Therapeutic Kinase Targets of Ulcerative Colitis; Authors - Sifan Tao
human,mouse	WB,IHC,IF	iScience; Inhibition of RIPK1 alleviating vascular smooth muscle cells osteogenic transdifferentiation via Runx2; Authors - Yue Li
mouse	IHC	World J Gastroenterol; OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex; Authors - Nan Wang
mouse	WB,IHC	Int J Biol Macromol; Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1; Authors - Hui-Wen Chiu