RIOK2 Polyclonal antibody

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  WB analysis using 17410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17410-1-AP (RIOK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 17410-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RIOK2 antibody (17410-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RIOK2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17410-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Serine/threonine-protein kinase RIO2, RIO2, RIO kinase 2, EC:2.7.11.1

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Tested Applications

• Positive WB detected in: Caco-2 cells, HCT 116 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17410-1-AP targets RIOK2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIOK2 fusion protein Ag9975
• Full Name: RIO kinase 2 (yeast)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC000953
• Gene Symbol: RIOK2
• Gene ID (NCBI): 55781
• RRID: AB_2178093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BVS4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rio-kinase 2 (RIOK2) is an atypical serine-threonine kinase that participates in the nuclear export and maturation steps of the pre-40S ribosomal complex to facilitate cytoplasmic translation. RIOK2 binds the pre-40S subunit and promotes its nuclear export by interaction with the CRM1 chaperone protein (PMID: 36661680, 28499923).