RIOK1 Polyclonal antibody

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  WB analysis of HeLa using 17222-1-AP

  WB result of RIOK1 antibody (17222-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIOK1 transfected HeLa cells.

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  WB analysis of HeLa using 17222-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 17222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17222-1-AP (RIOK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17222-1-AP

  IP result of anti-RIOK1 (IP:17222-1-AP, 4ug; Detection:17222-1-AP 1:500) with HeLa cells lysate 3200ug.

• Featured Product
• KD/KO Validated

RIOK1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17222-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

AD034, EC:2.7.11.1, RIO kinase 1, RIO1, Serine/threonine-protein kinase RIO1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIOK1 fusion protein Ag11057
• Full Name: RIO kinase 1 (yeast)
• Calculated Molecular Weight: 568 aa, 66 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC006104
• Gene Symbol: RIOK1
• Gene ID (NCBI): 83732
• RRID: AB_2284990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRS2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)

Publications

Species	Application	Title
human	WB	Biomedicines; RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.; Authors - Min Yu
human	WB	iScience; Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion.; Authors - Laura S Bisogno
human	WB	Cancer Res Treat; Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression.; Authors - Zhiqi Huang