RINT1 Recombinant antibody

RINT1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241592C4

Cat no : 84290-6-RR

Synonyms

RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1, HsRINT 1

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Validation Data Gallery

• 

  WB analysis of rat brain using 84290-6-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 84290-6-RR (RINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84290-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84290-6-RR against Human RINT1 were performed. The affinity constant is 0.551 nM.

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Tested Applications

• Positive WB detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84290-6-RR is RINT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RINT1 fusion protein Ag6145
• Full Name: RAD50 interactor 1
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC068483
• Gene Symbol: RINT1
• Gene ID (NCBI): 60561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.