RIMBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RIMBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15716-1-AP

Synonyms

KIAA0318, RBP2, RIM BP2, RIMBP2, RIMS binding protein 2

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Validation Data Gallery

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  WB analysis using 15716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15716-1-AP (RIMBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15716-1-AP (RIMBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15716-1-AP targets RIMBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIMBP2 fusion protein Ag8390
• Full Name: RIMS binding protein 2
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC007632
• Gene Symbol: RIMBP2
• Gene ID (NCBI): 23504
• RRID: AB_2878173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The presynaptic active zone mediates synaptic vesicle exocytosis at a synapse. RIM proteins are active zone scaffolding molecules that--among others--mediate vesicle priming and directly or indirectly interact with most other essential presynaptic proteins. RIMBP2 encodes RIMS-binding protein 2 belonging to RIMBP family. RIMBP2 is supposed to be localized at cell membrane especially synaptic plasma membrane. By interacting with RIMS1, RIMS2, CACNA1D and CACNA1B via the SH3 domains, RIMBP2 mediates synaptic transmission as bifunctional linker.

Publications

Species	Application	Title
mouse	WB	Elife; Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.; Authors - Fushun Fan ; KD Validated
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao