RILPL1 Recombinant antibody

• Featured Product
• KD/KO Validated

RILPL1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230515D1

Cat no : 83220-6-RR

Synonyms

RLP1, RILP-like protein 1, RILP like protein 1, Rab-interacting lysosomal-like protein 1, FLJ39378

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Validation Data Gallery

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  WB analysis using 83220-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83220-6-RR (RILPL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83220-6-RR

  WB result of RILPL1 antibody (83220-6-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILPL1 transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 83220-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83220-6-RR against Human RILPL1 were performed. The affinity constant is 1.97 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83220-6-RR is RILPL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RILPL1 fusion protein Ag10192
• Full Name: Rab interacting lysosomal protein-like 1
• Calculated Molecular Weight: 252 aa, 29 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC080626
• Gene Symbol: RILPL1
• Gene ID (NCBI): 353116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab effector-related proteins Rab-interacting lysosomal protein-like 1 (Rilpl1) and Rilpl2 regulate protein localization in the primary cilium. Rilpl2 was initially identified as up-regulated in ciliating mouse tracheal epithelial cells. Rilpl1 and Rilpl2 both localize to the primary cilium and centrosome, Rilpl1 specifically to the distal end of the mother centriole.(PMID:23264467)