RIG-1/DDX58 Recombinant antibody

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  WB analysis of A431 using 84861-5-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84861-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84861-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84861-5-RR against Human RIG-1/DDX58 were performed. The affinity constant is below 1 pM.

RIG-1/DDX58 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84861-5-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: A431 cells, THP-1 cells, HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84861-5-RR targets RIG-1/DDX58 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RIG-1/DDX58 fusion protein Ag18585
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Calculated Molecular Weight: 925 aa, 106 kDa
• Observed Molecular Weight: 101~106 kDa
• GenBank Accession Number: BC132786
• Gene Symbol: RIG-1/DDX58
• Gene ID (NCBI): 23586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O95786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.