RIC8B Polyclonal antibody

RIC8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17790-1-AP

Synonyms

Synembryn-B, RIC8, Protein Ric-8B, hSyn, Brain synembryn

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Validation Data Gallery

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  WB analysis of human brain using 17790-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17790-1-AP (RIC8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17790-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17790-1-AP (RIC8B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17790-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17790-1-AP (RIC8B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17790-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17790-1-AP (RIC8B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 17790-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 17790-1-AP (RIC8B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17790-1-AP targets RIC8B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RIC8B fusion protein Ag11888
• Full Name: resistance to inhibitors of cholinesterase 8 homolog B (C. elegans)
• Calculated Molecular Weight: 495 aa, 56 kDa
• Observed Molecular Weight: 61-66 kDa
• GenBank Accession Number: BC034220
• Gene Symbol: RIC8B
• Gene ID (NCBI): 55188
• RRID: AB_2285188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ric8, also known as synembryn, is a guanine nucleotide exchange factor (GEF) that enhances variegated G protein signaling pathways, including Gα(q) and Gα(s) regulation of neurotransmission, Gα(i)-dependent mitotic spindle positioning during (asymmetric) cell division, and Gα(olf)-dependent odorant receptor signaling (PMID: 21467038). In mammals, two Ric8 homologues have been reported (Ric8A and Ric8B). Ric8B is able to potentiate G(s)/G(olf) signaling (PMID: 15829631; 16754875; 20133939).

Publications

Species	Application	Title
mouse	WB,IHC	Front Cell Neurosci; Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling.; Authors - Fenech Claire C
mouse	WB	Sci Rep; The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia.; Authors - Stefan Kurtenbach
mouse	IF	PLoS One; Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis.; Authors - Merly Saare