RIC8A Monoclonal antibody

RIC8A Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E3B11

Cat no : 66625-1-Ig

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

---

Validation Data Gallery

• 

  WB analysis of HeLa using 66625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-Ig (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 66625-1-Ig

  IP result of anti-RIC8A (IP:66625-1-Ig, 5ug; Detection:66625-1-Ig 1:2000) with HEK-293 cells lysate 4000 ug.

• 

  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66625-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66625-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66625-1-Ig targets RIC8A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RIC8A fusion protein Ag1593
• Full Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011821
• Gene Symbol: RIC8A
• Gene ID (NCBI): 60626
• RRID: AB_2881985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Publications

Species	Application	Title
human	WB	J Clin Invest; Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies; Authors - Gonzalo P Solis