RHOT2 Monoclonal antibody

RHOT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C2

Cat no : 68469-1-Ig

Synonyms

ARHT2, C16orf39, hMiro 2, MIRO 2, Mitochondrial Rho GTPase 2, RASL, RHOT2

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Validation Data Gallery

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  WB analysis using 68469-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68469-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: U2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68469-1-Ig targets RHOT2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHOT2 fusion protein Ag29690
• Full Name: ras homolog gene family, member T2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC014942
• Gene Symbol: RHOT2
• Gene ID (NCBI): 89941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHOT2 (Mitochondrial Rho GTPase 2) is also named as MIRO-2, hMiro-2, ARHT2, C16orf39 and Ras homolog gene family member T2. RHOT2 belongs to the mitochondrial Rho GTPase family. RHOT2 gene encodes a member of Rho family of GTPase, which are localized to the outer membrane of mitochondria (PMID: 22078885).