RHOG-Specific Polyclonal antibody

RHOG-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19671-1-AP

Synonyms

ARHG, RHOG, RHOG-Specific

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 19671-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 19671-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 19671-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19671-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19671-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, C6 cells, HeLa cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19671-1-AP targets RHOG-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ras homolog gene family, member G (rho G)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: NM_001665
• Gene Symbol: RHOG
• Gene ID (NCBI): 391
• RRID: AB_2878593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.