RHOG Polyclonal antibody

RHOG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21576-1-AP

Synonyms

ARHG, RHOG

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Validation Data Gallery

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  WB analysis using 21576-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21576-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21576-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21576-1-AP is RHOG Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOG fusion protein Ag16118
• Full Name: ras homolog gene family, member G (rho G)
• Calculated Molecular Weight: 191 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC104178
• Gene Symbol: RHOG
• Gene ID (NCBI): 391
• RRID: AB_2878887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.