RHOF Polyclonal antibody, PBS Only

RHOF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12290-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12290-1-AP (same clone as 12290-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12290-1-AP (same clone as 12290-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12290-1-AP (same clone as 12290-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 12290-1-AP (same clone as 12290-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IP experiment of COLO 320 using 12290-1-AP (same clone as 12290-1-PBS)

  IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug. This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 12290-1-AP (same clone as 12290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12290-1-PBS in a different storage buffer formulation.

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Product Information

12290-1-PBS targets RHOF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RHOF fusion protein Ag2943
• Full Name: ras homolog gene family, member F (in filopodia)
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC018208
• Gene Symbol: RHOF
• Gene ID (NCBI): 54509
• RRID: AB_10644126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.